MatchaDB

2018Journal articleFree to read

Effect of powdered green tea matcha on biofilm formation by mutans Streptococci

Mochamad Fahlevi Rizal, Noboru Kaneko, Hiroshi Ogawa

STOMATOLOGY EDU JOURNAL

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Abstract

Introduction: Antibacterial ingredients with high safety and mild taste that can be used for mouthwash for children are required.Matcha is one of the natural beverages that is made from the leaves of Camellia sinensis and widely consumed by Japanese and Asian people.This study aimed to evaluate the effectiveness of matcha in suppressing biofilm formation by mutans streptococci, typical cariogenic bacteria.Methodology: Five laboratory strains of Streptococcus mutans (serotype c, e and f ) and Streptococcus sobrinus (serotype d and g) were used to evaluate the antibacterial effect of matcha.Matcha extract was added to bacterial cells in Heart Infusion broth supplemented with 1% sucrose (HIS).After incubation for 24 hours, the formed biofilm was dyed by Crystal Violet, and optical density at 490 nm was determined as the amount of biofilm formation.The effect of 0.02% chlorhexidine in HIS and MilliQ in HIS were also measured as well.Results: The amounts of biofilm formation by all serotypes of mutans streptococci in matcha + HIS were significantly lower than those in MilliQ + HIS as were chlorhexidine in HIS, except S. sobrinus serotype g.The differences of amounts biofilm formation by all S. mutans (serotype c, e and f ) and S. sobrinus serotype d in matcha + HIS and in chlorhexidine + HIS were not significant statistically.Conclusion: Matcha extract has an equivalent effectiveness of 0.02% chlorhexidine against most serotypes of mutans streptococci.

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Study type

Food science / processing

Making, milling, storing or using matcha in foods.

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Keywords and subject terms

Cite this study

Mochamad Fahlevi Rizal, Noboru Kaneko, Hiroshi Ogawa. (2018). Effect of powdered green tea matcha on biofilm formation by mutans Streptococci. STOMATOLOGY EDU JOURNAL. https://doi.org/10.25241/stomaeduj.2018.5(4).art.2

DOI: 10.25241/stomaeduj.2018.5(4).art.2

More on inflammation and immunity

2020Journal articleFree to read

Health Benefits and Chemical Composition of Matcha Green Tea: A Review.

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Japanese matcha is a type of powdered green tea, grown in a traditional way. Shading of the plants during the growth period enhances the processes of synthesis and accumulation of biologically active compounds, including theanine,…

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2019Journal articleFree to read

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Schröder L, Marahrens P, Koch JG, et al. — Oncology reports

We investigated the anticarcinogenic potential of green tea and its components epigallocatechin gallate (EGCG) and quercetin, as well as tamoxifen, on MCF-7 and MDA-MB-23 breast cancer cells. Using high-performance liquid chromatography,…

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2021Journal articleFree to read

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Zhou J, Yu Y, Ding L, et al. — Nutrients

Lately, matcha green tea has gained popularity as a beverage and food additive. It has proved to be effective in preventing obesity and related metabolic syndromes. However, the underlying mechanisms of its control effects against…

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2021Journal articleFree to read

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Kim JM, Kang JY, Park SK, et al. — Antioxidants (Basel, Switzerland)

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