MatchaDB

2023Preprint

Effects of matcha tea extract on cell viability and estrogen receptor β expression on MCF7 breast cancer cells

Keckstein S, Tilgener C, jeschke u, Hofmann S, Vilsmaier T, Keilmann L, Heidegger H, Kaltofen T, Batz F, Mahner S, Schröder L

Research Square

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Abstract

Abstract Purpose: In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor beta expression (ERβ). Methods: MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a WST-1 assay after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results: The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast the PCR did not result in significant downregulation of ERβ. Conclusion: MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level.

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Study type

Chemical / analytical

Measuring what is in matcha, or how to detect it.

Assigned automatically from the title, abstract and subject terms.

Topics

Cancer

Matcha is a main subject here (named in the title).

Cite this study

Keckstein S, Tilgener C, jeschke u, Hofmann S, Vilsmaier T, Keilmann L, … Schröder L. (2023). Effects of matcha tea extract on cell viability and estrogen receptor β expression on MCF7 breast cancer cells. Research Square. https://doi.org/10.21203/rs.3.rs-3123368/v1

DOI: 10.21203/rs.3.rs-3123368/v1

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